Biosynthesis of Sclareol, β-Sitosterol, and Oleanolic Acid from Mevalonic Acid-2-C14

Biosynthesis of Sclareol, p-Sitosterol, and Oleanolic Acid from Mevalonic Acid-2-C”*

In previous studies of the incorporation of sodium acetateZ-Cl4 (1, 2) and sodium mevalonate-2-C14 (2) into &sitosterol and the acidic triterpenes of Salvia oficinalis, Cl4 from both precursors was found in sterol and triterpenes. The presence of sclareol (Fig. l), /3-sitosterol, oleanolic acid, and ursolic acid II (3, 4) in the plant Xalvia sclarea has afforded an opportunity to duplicate thes...

The biosynthesis of mevalonic acid from 1-C14-acetate by a rat liver enzyme system.

Although it has been conclusively demonstrated in numerous reports that acetate and mevalonic acid are converted to squalene or sterols (3-lo), it has not been proved that mevalonic acid is formed from acetate by a mammalian enzyme system. Evidence has been presented, however, for the formation of this compound from P-hydroxy, P-methyl glutaryl coenzyme A in the presence of reduced triphosphopy...

The Biosynthesis of Mevalonic Acid.

32 Fraenkel-Conrat, H., Biochim. Biophys. Acta, 15, 307 (1954). 33 Ochoa, S., Arch. Biochem. Biophys., 69, 119 (1957). 34 Grunberg-Manago, M., J. Molecular Biology (in press). 35 Kalnitsky, G., J. P. Hummel, C. Dierks, H. Resnick, L. B. Barnett and J. R. Carter, Ann. N.Y. Acad. Sci. (in press). 36 Rice, S. A., and P. Doty, unpublished work; S. A. Rice, Thesis, Harvard Univ., 1956. 37 James, T. ...

Biosynthesis of abscisic acid: incorporation of radioactivity from [2-14C]mevalonic acid by intact fruit.

The biosynthesis of glucuronic acid from 1-C14-glucose.

Although many classes of substances found in the animal organism have been suggested as possible biological precursors of glucuronic acid (l), recent research has narrowed the number of these possibilities to two, namely hexoses (2,3) and trioses (4, 5). Various experimental techniques, including isotopic tracers, have been used in the effort to choose between these alternatives, but invariably...